The Total Urine Cortisol test measures the total amount of cortisol excreted in the urine over a specified period, typically 24 hours. Cortisol is a hormone produced by the adrenal glands that helps regulate various body functions, including metabolism, immune response, and stress. This test is used to evaluate adrenal gland function and diagnose conditions such as Cushing’s syndrome (excess cortisol) or Addison’s disease (insufficient cortisol). Abnormal cortisol levels in the urine can help identify hormonal imbalances or disorders related to the adrenal glands.